Toxicology International (Formerly Indian Journal of Toxicology)

Reetu Bhanot ¹, Gurinder Kaur Sangha ²

Affiliations
1 Department of Zoology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana – 141004, Punjab, IN
2 Punjab Agricultural University, Ludhiana – 141004, Punjab, IN

Abstract

Triazophos (TZ) at the level of Acceptable Daily Intake (ADI) (0.01 mg/kg bw/day) and 10×ADI (0.1 mg/kg bw/day) was given to the female albino rats to examine its effects on their reproductive system. In this study, the pregnant female albino rats were divided into three groups, each group comprising of six rats. The first group was gavaged with olive oil (control group), the second group was administered with ADI level of TZ (ADI group) and the third group was treated with 10×ADI level of TZ (10×ADI group). The dose was given through oral intubation until the end of pregnancy. At the end of gestational period, the reproductive parameters of dams were examined, however, the females which did not breed were dissected for uterine examination. The results of the present study revealed non-significant decrease in the body weight gain of females treated with ADI and 10×ADI in comparison with controls. The analysis of reproductive index depicts significant decrease in percentage pregnancy, litter size and pup survival, however, significant increase in gestational length of ADI group females as compared to controls. The examination of organ weight indicated significant decrease in weight of liver, however, significant increase in weight of thyroid, ovary, uterus and oviduct of 10×ADI group females in comparison to control. The levels of biochemical constituents viz. protein and Alanine transaminase (ALT) were significantly increased in plasma, however, the levels of Alkaline Phosphatase (AKP) significantly increased in ovaries of 10×ADI group females as compared to control. Histological studies of ovaries revealed significantly enhanced percentage of follicular atresia, however, significantly decreased diameter of ovarian follicles in 10×ADI group females. Histological examination of liver indicates distorted central vein, vacuolization of hepatocytes, dilated sinusoids with intense mono nuclear inflammatory cells and infiltration in ADI and 10×ADI group females as compared to controls. Hence, in utero intoxication of female rats with TZ at the level of ADI and 10×ADI has a potential to induce alterations at cellular and organ level.

Keywords

Females, in Utero, Reproduction, Rattus Norvegicus, Triazophos.

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